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| Registros recuperados: 18 | |
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| Rubim,Alexandre Machado; Rubenick,Jaqueline Bandeira; Laporta,Luciane Varine; Rolim,Clarice Madalena Bueno. |
| A rapid, simple and low cost method was developed to determine diclofenac potassium (DP) in oral suspension, using a reverse-phase column (C8, 150 mm x 4.6 mm, 5 µm), mobile phase containing methanol/buffer phosphate (70:30 v/v, pH 2.5), at a flow rate of 1.0 mL/min, isocratic method, and ultraviolet detection at 275 nm. A linear response (r = 1.0000) was observed in the range of 10.0-50.0 µg/mL. Validation parameters such as linearity, specificity, precision, accuracy and robustness were evaluated. The method presented precision (repeatability: relative standard deviation = 1.21% and intermediate precision: between-analyst = 0.85%). The specificity of the assay was evaluated by exposure of diclofenac potassium under conditions of stress such as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: High performance liquid chromatography; Diclofenac potassium; Oral suspension. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300021 |
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| Sawaya,Alexandra C. H. F.; Souza,Kirllian S.; Marcucci,Maria C.; Cunha,Ildenize B.S.; Shimizu,Mario T.. |
| Brazilian propolis from São Paulo state was submitted to extraction using several solvents, resulting in extracts with different composition. These extracts were submitted to Thin Layer Chromatography (TLC). Bioauthographic analysis of the TLC plates identified fractions with inhibitory activity, which were then analysed by High Performance Liquid Chromatography (HPLC). In vitro assays, commonly used to evaluate the activity of propolis against Gram-positive bacteria, were compared to determine which rendered the most consistent results. The bactericidal activity of these extracts were analysed by Serial Dilution in Tubes and Agar Plate Diffusion. Serial Dilution in Tubes obtained the most consistent results, with the Minimal Bactericidal Concentration of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Propolis; Thin layer chromatography; Bioauthography; High performance liquid chromatography; Extraction methods; Minimal bactericidal concentration. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100017 |
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| Vicentini,Fabiana Testa Moura de Carvalho; Georgetti,Sandra Regina; Bentley,Maria Vitória Lopes Badra; Fonseca,Maria José Vieira. |
| To be effective against the oxidative damages induced by UVB irradiation in the skin, the drug needs to release from the formulation in which it was incorporated and reach the skin layers where the ROS are generated. Thus, it is very important the development of a robust and sensitive methodology to extract and quantify in different skin layers the antioxidant agent delivered from topical formulations. Therefore, in the present work suitable methods to extract and quantify quercetin in skin samples and receptor phase after in vitro penetration studies were developed. The results demonstrated that the recovery from two different layers of skin, the SC and [E+D], using two different methods of quantification (DPPH assay and HPLC, respectively), was 93.8 %... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Topical formulations/analysis; Quercetin/quantification; Quercetin/extraction; Quercetin/liberation; Skin penetration in vitro/study; High performance liquid chromatography; DPPH. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200022 |
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| Ferreira,Márcia Pires; Oliveira,Maria Cristina Neves de; Mandarino,José Marcos Gontijo; Silva,Josemeyre Bonifácio da; Ida,Elza Iouko; Carrão-Panizzi,Mercedes Concórdia. |
| The objective of this work was to analyze changes in the isoflavone profile, determined by high performance liquid chromatography, at different processing stages and after refrigeration of tempeh. For tempeh production, clean soybean grains from cultivars BR 36 (low isoflavone content) and IAS 5 (high) were dehulled, and the separated cotyledons were hydrated and then cooked in boiling water for 30 min. Spores of the fungus Rhizopus microsporus var. oligosporus were inoculated in the cooked and cooled cotyledons, and incubated at 32ºC for 6, 12, 18, and 24 hours in perforated polypropylene bags, for fermentation. The resulting tempeh was stored at 4ºC for 6, 12, 18, and 24 hours. After 24-hour fermentation, isoflavone glucosides were 50% reduced, and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rhizopus microsporus var. oligosporus; Aglycone; Fermentation; Functional food; High performance liquid chromatography; Malonyl. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011001100018 |
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| Queiroz,Kelly Marques; Silva,Maria-Luiza Martins; Prado,Nathália Duque; Lima,Paulo Marcelo Andrade; Diniz,Rosiane Dias Lopes; César,Isabela Costa; Pianetti,Gerson Antônio; Santos,Daniel Assis. |
| The development of a specific agar diffusion bioassay for the quantitative determination of fluconazole formulated in capsules was carried out using a strain of Candida albicans ATCC 18804 as the test organism. A prospective validation of the method showed adequate linearity (r²=0.9995), precision (R.S.D. = 4.0% for intra-day and 4.5% for inter-day precision) and accuracy (mean recovery = 102.9%). High performance liquid chromatography was chosen as a comparison method for the fluconazole determination. The contents of fluconazole determined by both methods, for four capsule samples, showed a strong correlation, confirmed by Pearson's correlation coefficient value (r = 0.9884). The bioassay is a suitable method for both research and pharmaceutical industry... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fluconazole/quantitative determination; Microbiological assay/agar diffusion; High performance liquid chromatography. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400012 |
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| Codevilla,Cristiane Franco; Tischer,Bruna; Gindri,Amanda Leitão; Nogueira-Librelotto,Daniele Rubert; Barin,Juliano Smanioto; Silva,Cristiane de Bona da; Rolim,Clarice Madalena Bueno; Zepka,Leila Queiroz; Menezes,Cristiano Ragagnin de. |
| ABSTRACT: The high intensity ultrasound-assisted extraction (HIU) is one of the most simple, quick and efficient techniques for the extraction of phenolic and other antioxidant compounds from plants. This is the first application of HIU for the extraction of these compounds from goldenberry fruit. The HIU and conventional extraction techniques showed similar results regarding to phenolic compounds and antioxidant capacity. However, the time required for HIU extraction (5min) was 24 times lower than conventional extraction (120min). Phenolic compounds reported were chlorogenic acid, caffeic acid and rutin. In vitro cytotoxicity assays were used for evaluation of extracts and the results showed that in a wide range of concentration, the extract maintains... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goldenberry; Sonication; Antioxidant capacity; Cytotoxicity; High performance liquid chromatography. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200752 |
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| ROSA, J. S. da; FREITAS-SILVA, O.; CAMPOS, R. da S.; CASTRO, I. M. de; TEIXEIRA, A. da S.. |
| O café é a segunda maior commodity do mundo e tamanha importância faz do café uma matriz alimentar muito estudada. A necessidade de incremento na produtividade dessa cultura, porém, pode aumentar o uso de alguns agrotóxicos em sistemas de produção de café. Da mesma forma, as práticas de colheita e processamento pós-colheita do café estão diretamente relacionadas à contaminação dos grãos por fungos micotoxigênicos. Rastrear a presença destes dois tipos de contaminantes em amostras de café Arábica comum (com diferentes teores de grãos defeituosos) e submetidos a diferentes níveis de torra foi o objetivo deste trabalho. Os agrotóxicos foram analisados pelo método multirresíduo, por espectrometria de massas sequencial, e a ocratoxina A, por cromatografia... |
| Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) |
Palavras-chave: Contaminante; Cromatografia líquida de alta eficiência; Micotoxina; Coffea Arábica; Tecnologia de Alimento; Espectrometria; Cromatografia; Food technology; Vegetable products; High performance liquid chromatography; Mycotoxins; Mass spectrometry; Poisoning; Agrochemicals. |
| Ano: 2019 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1112112 |
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| Aguiar,José Luiz Neves de; Leandro,Katia Christina; Abrantes,Shirley de Mello Pereira; Albert,André Luis Mazzei. |
| Acetylsalicylic acid (AAS) is a drug utilized as analgesic, anti-inflammatory, and antipyretic medication, available worldwide and commonly used in Brazil. Salicylic acid (AS) is a precursor in AAS synthesis and is also produced during its degradation. The official United States Pharmacopoeia (USP) suggests the determination of these drugs by high performance liquid chromatography (HPLC), with ultraviolet detection, but this method has neither a high sensitivity (S AAS=0.12 mAbs/(μg/mL) and S AS=0.48 mAbs/(μg/mL)) nor resolution (Rs=1.61). The purpose of this study was to develop a new more adequate, accurate method by liquid phase chromatography than the current official methodology, and to use this new method in the determination of the tenors of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: High performance liquid chromatography; Acetylsalicylic acid/determination in tablets; Salicylic acid/determination in tablets; Tablets/quantitative analysis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400016 |
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| Fregonezi-Nery,Marlene Maria; Prudencio-Ferreira,Sandra Helena; Kedor,Érika Maria Rosa; Baracat,Marcela Maria; Brinholi,Francis Fregonezi. |
| Sensory analysis was used in the albendazole suspension stability study. Three formulations were prepared and stored for 1, 3, and 6 months at 4 ±1, 26 ±1, 37, 50 and 65 ºC. Samples were evaluated through the difference from control sensory test using 24 trained judges in individual cabins. Although albendazole content was not altered in the conditions studied, sensory test showed differences between control and stored samples, except for one of the formulations stored under refrigeration for one month. These results have shown that the sensory evaluation is an important tool for quality control of pharmaceutical preparations, in association with chemical, physicochemical and microbiological tests. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Albendazole; Sensory analysis; High performance liquid chromatography. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000600009 |
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| Pakinee Akkaravessapong; Suganya Wongpornchai; Sunanta Wongpiyachon; Kitsada Pitija; Angsutorn Wasusun; Panawan Suttiarporn. |
| Rice contains several kinds of bio-functional components. In this study, γ-aminobutyric acid (GABA) and Tocopherol, or Vitamin E were identified and their relationships to antioxidant activity using DPPH assay of both brown and pre germinated Thai rice varieties were studied. GABA are one of the two inhibitory neurotransmitters which make the neuron resistant to stimulation by excitatory neurotransmitters and inhibitor of some cancer cells activity whereas Tocopherol, or Vitamin E, which acts as antioxidant and improve blood cholesterol. Identification of GABA and Tocopherol in both brown and pre-germinated brown rice grown from different locations were conducted in eleven varieties, namely: Khao Dawk Mali 105, PTT1, CN1, RD31, Sang yod, RD6, Hom... |
| Tipo: PhysicalObject |
Palavras-chave: Antioxidant activity; Rice varieties; Tocopherol; DPPH assay; Brown rice; Pre-germinated brown rice; GABA; Gamma aminobutyric acid; Vitamin e; Nutritive value; Chemical composition; High performance liquid chromatography; HPLC; ฤทธิ์ต้านอนุมูลอิสระ; กาบา; โทโคฟีรอล; พันธุ์ข้าว; ข้าวกล้อง; ข้าวกล้องงอก; วิตามินอี; โครงสร้างทางเคมี; การวิเคราะห์องค์ประกอบทางเคมี; คุณค่าทางโภชนาการ. |
| Ano: 2013 |
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5713 |
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| Registros recuperados: 18 | |
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